Yeast Two-Hybrid

This technology is based on the fact that the activating domain (AD) and binding domain (BD) of a transcription factor can be separated and still function when they get into close proximity. Y2H screening is therefore designed to use a BD fused bait protein to screen against a library of AD fused prey proteins. The bait is usually a known protein that the researcher tries to identify new interacting partners with. The prey is usually a library of proteins to be screened from. When the bait protein binds to the prey protein, transcription of the specially designed reporter genes occurs and allows selection of this positive clone. Profacgen use modified yeast strains which incorporates several reporter genes to reduce false positives. We also use different GAL4 binding elements to increase sensitivity of the assay.