Replication-Competent Lentivirus Testing

Lentiviral vectors have demonstrated great potential as gene therapy vectors mediating efficient in vitro and in vivo gene delivery and long-term transgene expression in both dividing and nondividing cells. They have the advantages of a large cloning capacity, the lack of a requirement for viral gene expression, and the capacity to integrate into the target cell genome for stable transduction. In addition, they are highly efficient for in vivo gene delivery in a broad range of hosts cells, such as neurons, endothelial cells, hepatocytes, and hematopoietic stem cells.