Recombineering Microbe

Recombinase-mediated homologous recombination, also known as recombineering, has revolutionized bacterial genetics, synthetic biology, and metabolic engineering. Relying on either RecET from the Rac prophage or the bacteriophage lambda Red proteins, Exo, Beta, and Gam, recombineering allows simple and efficient construction of gene knockout mutants via homologous recombination of a double stranded DNA (dsDNA) polymerase chain reaction (PCR) product with bacterial chromosomes.