Protein Structure

Because there is no digestion of proteins before analysis, the information which is lost when shotgun method is used, for example, the quaternary structure with disulfide bonds, can be maintained and detected when the Top-Down strategy is applied, which show unique advantage in protein-level characterization of disulfide bonds. This make it get more and more application in QC analysis of protein therapeutics, such as insulin, to make the recombinant protein are correctly expressed, folded and assembled.