Peptide Nucleic AcidPharmaceutical InformationUpdated on Feb 26, 2019 View more like this | Visit SHIRLEY, NY | Contact Cathy Miller |
Creative Peptides can provide a number of PNA related products, such as Gamma PNA or Fmoc Monomers etc., which are commonly and widely used in below listed areas.
•Microarrays and biosensors — PNA microarray combined with PCR could detect genetically modified organisms.
•PNA FISH Probes — In fluorescent in situ hybridization (FISH), short probes can be difficult to detect (owing to insufficient hybridization and low levels of labelling), while longer probes can induce non-specific background fluorescence. In PNA-FISH, shorter PNA probes can be used (because of the higher specificity of PNA), resulting in less background noise.
•PCR Blockers — PNA probes have no direct interaction with DNA polymerase but PNAs can terminate the elongation of oligonucleotide primers by binding to the template or competing with the primers. Moreover, PNA–DNA chimeras can be recognized by the DNA polymerase and can thus be used as primers for PCR reactions. The high affinity binding of PNAs has also been used for detecting single base pair mutations by PCR. This strategy, named PNA directed PCR clamping, uses PNAs to inhibit the amplification of a specific target by direct competition of the PNA targeted against one of the PCR primer sites and the conventional PCR primer.
•Microarrays and biosensors — PNA microarray combined with PCR could detect genetically modified organisms.
•PNA FISH Probes — In fluorescent in situ hybridization (FISH), short probes can be difficult to detect (owing to insufficient hybridization and low levels of labelling), while longer probes can induce non-specific background fluorescence. In PNA-FISH, shorter PNA probes can be used (because of the higher specificity of PNA), resulting in less background noise.
•PCR Blockers — PNA probes have no direct interaction with DNA polymerase but PNAs can terminate the elongation of oligonucleotide primers by binding to the template or competing with the primers. Moreover, PNA–DNA chimeras can be recognized by the DNA polymerase and can thus be used as primers for PCR reactions. The high affinity binding of PNAs has also been used for detecting single base pair mutations by PCR. This strategy, named PNA directed PCR clamping, uses PNAs to inhibit the amplification of a specific target by direct competition of the PNA targeted against one of the PCR primer sites and the conventional PCR primer.
