mass spectrometric analysis

Peptide Mass Fingerprinting (PMF) assay is a method for mass spectrometric analysis of a mixture of polypeptides obtained after proteolysis or degradation. The peptide obtained by mass spectrometry is compared with the theoretical peptide of the protein in the polypeptide protein database to determine whether the measured protein is known or unknown. Since different proteins have different amino acid sequences, the peptides obtained from different proteins have fingerprint characteristics. The absolute mass of each protein peptide is theoretically calculated to find the best match compared to the mass of the unknown protein peptide. A variety of proteomes have been studied using peptide fingerprinting methods.