Large-scale Protein Production

Large-scale recombinant protein production is becoming increasingly important for applications in the field of proteomics, structural biology and protein therapeutics. When large quantities of protein (from mg to g amounts) needs to be produced from either bacterial (e.g. Escherichia coli) or eukaryotic cell-based culture (e.g. yeast, insect and mammalian cell), cells must be cultured in large volume by the implementation of fermentation techniques. Fermentation can improve efficiency by increasing cell density and provide easy-to-handle large culture over 4 liters. Depending on the high cell density as well as fermentation strategy, we can optimize cell culture conditions to increase cell mass and finally improve recombinant protein yield.