Isocitrate Dehydrogenase (IDH)

Isocitrate dehydrogenase (IDH) is an important enzyme in the tricarboxylic acid cycle, which occurs in the mitochondrial matrix. IDH is responsible for catalyzing the reversible conversion of isocitrate to alpha-ketoglutarate and CO2 in a two-step reaction. The first step of the reaction involves the oxidation of isocitrate to the intermediate oxalosuccinate. The second step of the reaction looses the beta-carboxylate of the oxalosuccinate intermediate as carbon dioxide leaving alpha-ketogluterate. During the catalyzation of isocitrate to alpha ketoglutarate either NADH or NADPH is produced along with carbon dioxide.