Identification of protein gel samplePharmaceutical InformationUpdated on Aug 28, 2019 View more like this | Visit SHIRLEY, NY | Contact Cathy Miller |
Peptide Mass Fingerprinting (PMF) assay is a method for mass spectrometric analysis of a mixture of polypeptides obtained after proteolysis or degradation. The peptide obtained by mass spectrometry is compared with the theoretical peptide of the protein in the polypeptide protein database to determine whether the measured protein is known or unknown. Since different proteins have different amino acid sequences, the peptides obtained from different proteins have fingerprint characteristics. The absolute mass of each protein peptide is theoretically calculated to find the best match compared to the mass of the unknown protein peptide. A variety of proteomes have been studied using peptide fingerprinting methods.
The advantage of PMF is that if the protein is present in the protein database, only the mass of the protein peptide is known, and it takes no time to perform DEVONO sequencing. However, PMF-based protein identification relies on a single protein because mixed proteins can significantly complicate PMF analysis and may yield incorrect results. Therefore, for PMF identification with more than 2-3 protein samples, additional MS/MS-based protein identification is required to enhance the specificity of protein identification.
The advantage of PMF is that if the protein is present in the protein database, only the mass of the protein peptide is known, and it takes no time to perform DEVONO sequencing. However, PMF-based protein identification relies on a single protein because mixed proteins can significantly complicate PMF analysis and may yield incorrect results. Therefore, for PMF identification with more than 2-3 protein samples, additional MS/MS-based protein identification is required to enhance the specificity of protein identification.
