humanised monoclonal antibody

This method allows selection of humanized antibodies in a full-size IgG format that retain or increase the original affinity of the mouse antibody. Also, in comparison with the above elaborated bacterial phage display based methods, this mammalian cell surface display approach allows selection of high affinity humanized antibodies in a dimeric IgG format; selected IgG are immediately good for further downstream applications—avoiding time consuming conversion of scFv to IgG and codon optimization of converted IgG (for expression in mammalian cells), which sometime deselect some humanized scFvs. Also, some good antibodies have sequences that prevent protein synthesis and phage display in bacterial cells; these problems can be well overcome in this exclusively mammalian cells based system.