Histone Demethylase Screening

Demethylases are a kind of enzymes that remove methyl groups from lysine and arginine residues of proteins, and currently the most commonly studied are histone demethylases. Histones’ methylation is essential for modulating transcription and maintaining chromatin structure. And demethylases play an important role in development and gene regulation just like all other histone modifying enzymes. Except LSD1A, all of the other demethylases contain a catalytic jumonji domain. Many of these demethylases require a cofactor such as FAD, Fe(II), or α-ketoglutarate.