Di-Sulfide Bond Localization

Disulfide bond is a type of post-translational modifications in proteins formed between the sulfur atoms of two cysteine residues during the biosynthesis of the proteins in the cell. Disulfide bonds are important in protein folding, they play a significant role in both protein structure and function. Therefore, the analysis of disulfide bond in proteins is of great significance to reveal the higher structure and biological functions of proteins. In addition, incorrect disulfide bond formation or exchange can cause antibody aggregation, so understanding the disulfide bonding is critical for protein characterization in the biopharmaceutical production process.