car plasmids

Aiming at genetically modifying T cells with designed CAR, our scientists flexibly adopt versatile methodologies to transfect CAR construction into T cells, such as packing and transfection with retrovirus or lentivirus. Since the successful manufacture of CAR-T cells is largely dependent on T cell receptor (TCR) gene transfer, this genetic modification of T lymphocytes is one of the most critical steps to generate superior CAR-T cells. Although the T cells are difficult to modify using non-viral methods like lipid-based car t plasmid transfection due to the high toxicity and low efficiency, the electroporation and several virus-based transfection systems are much more robust to complete the T-cell transfection.