antibody avidity

We offer Biacore Analysis services for binding kinetic analyses of antibodies. We typically capture the antibody on the chip and run antigen on top of the captured antibody. The antigen will be ran at 6 different concentrations for each antibody and chi-square analysis will be performed on the binding constants we obtain from each antigen concentration. The documentation package will include a real time on-rate (Ka), off rate (Kd), an affinity constant (KD), chi square value and a graph of real-time binding kinetics. We would like to obtain ~50 uL of 1 mg/mL antigen and antibody solutions. We will need ~100 ug of antigen and ~50ug for each antibody. We would need MW information for the antigen as well. It may require special considerations for antigens with repeated or multiple epitopes for affinity determination.