ADA evaluation 

ADA screening assay: Screening assays are performed to identify ADA presence in the samples. Common methods include direct or bridging ELISA, ECL, RIA and Biacore. Non-specific background (NSB) is usually determined by testing couples of serum samples of untreated animals on several different days. Afterwards, the cut point of ADA assay is determined according to the NSB.
ADA confirmatory assay: Positive screened samples must be analyzed in a confirmatory assay to rule out any false-positive results.
ADA characterization assay: Confirmed positive samples will be further characterized for their binding affinity, isotyping, and other characteristics.